SGCA antibody (AA 217-289)

Catalog No. ABIN7538837

The Mouse Monoclonal anti-SGCA antibody has been validated for IHC and IHC (fro). It is suitable to detect SGCA in samples from Human.

Quick Overview for SGCA antibody (AA 217-289) (ABIN7538837)

Target: SGCA (Sarcoglycan, alpha (50kDa Dystrophin-Associated Glycoprotein) (SGCA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SGCA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Ad1-20A6

Product Details anti-SGCA Antibody

Binding Specificity: AA 217-289

Purpose: Liquid Mouse Monoclonal Antibody Alpha-Sarcoglycan (Adhalin)

Specificity: Human alpha-sarcoglycan, also known as adhalin. Also crossreacts strongly with alpha-sarcoglycan in sections of muscle from mouse, rat, rabbit, hamster and pig. Does not react with chicken muscle.

Characteristics: This product is intended for the qualitative identification by light microscopy of Alpha-Sarcoglycan (Adhalin) by immunohistochemistry.

Purification: Tissue culture supernatant

Immunogen: Fusion protein containing amino acids 217 to 289 of the rabbit adhalin sequence (Roberds SL et al., 1993).

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections. Suggested dilution: 1:100-1:200 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 58.5 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for SGCA

Target: SGCA (Sarcoglycan, alpha (50kDa Dystrophin-Associated Glycoprotein) (SGCA))

Alternative Name: Alpha-Sarcoglycan